QUALITY CONTROL FOR AVIAN CORYZA BACTERIN

            QUALITY CONTROL FOR AVIAN CORYZA BACTERIN

    

            Carlos Rodríguez G.   Biofarma  Laboratories  (carlosrodriguezg25@gmail.com) 2013

The quality control tests for Coryza bacterin are very similar if the chick embryo culture and broth propagated and are mandatory for each lot.

Titration

1.-A sample of 10 ml is sufficient crop. It is sown in blood agar, cross seeding with Staphilococcus epidermidis.(Mothership) Incubate for 24 hours.

2. - At the same time, proceed to do a titration, took a series of tubes containing 6 broth of Brain Heart Infusion each tube containing 4.5 ml.

3.-Take a sample of 0.5 ml per head and add it to the first tube, mixed with another different pipette entire contents of the tube for 5 times.

4.-We have the first dilution of 1 in 10 and if we continue with the remaining tubes, until finally discard 0.5 ml. we are diluting 10-1 to 10-6.

5.-Using another pipette start with the highest dilution for this previously frame with marker ink on the back a Petri containing blood agar, grids must be from the dilution 10-6 to 10-3.

6.-We placed a tuberculin syringe with 0. 0 1ml per frame starting with the highest dilution to 10-3 and discarding lowest end 0.5.

 left the petri culture, long enough to allow the drops to dry, and immediately after using a platinum loop, take the Mothership enough to make a grid on the agar

7.-incubate for 24 hours

TITULACIÓN

8. - At 24 hours there are colonies of each frame starting 10-6, this can be done with the naked eye or with an account colonies, colonies are added and removed an average, the number of colonies represents your titration in 10-6 for 0 0 1 ml, but as we give the title by 1ml,. runs until dilution is 10.8 ml.

9. - It is advisable to do this test in duplicate to avoid an error

There is a titration method that allows us to instantly know the number of organisms per ml. And it is used for monitoring the growth in our reactor, when we are producing the broth culture, it is not accurate but give us a rough idea of the titration

MacFarland Nephelometer consists of a series of tubes with a turbidity, which shows a greater concentration to less concentration.The technique for manufacturing a Nephelometer  comes widely explained on Clinical Diagnosis by Laboratory Metods by Todd, Sanford and Wells pp. 892-96. not explained in this article.

NEFELÓMETRO DE MACFARLAND

This comparative method lets us know that when we reach the maximum growth curve and is highly recommended for its simplicity and its result 

10.-Direct sowing was done on blood agar must be a pure culture, compared to a seed agar and brain heart infusion should leave negative AICC.

Inactivated bacterin When this test is safety, seeding AICC and brilliant green agar,

Result: After an incubation period of 48 hours, cultures must be negative.

11. - 0.1 ml is inoculated to 5 embryos from 10 days of incubation for 3 days, then make a hemagglutination test and observe if a retarded embryo size is not U-shaped or very closed, with this rule if there is some contamination newcastle virus, influenza or bronchitis

12. We do a power test, one ml subcutaneously vaccinated. bacterin to 10 chickens over 7 weeks old.

13.-A similar number of chickens served as controls and not vaccinated, both should be well insulated and separated from each other.

14. - Apply a eye drop of a pure culture containing a titre off 10-8  to all  chickens, vacunated and unvaccinated.

15. - At 48 hours sinuses are observed and if there is a rattling looking  both chickens vaccinated or not.

16. - The result will be effective if all are normal chickens vaccinated and unvaccinated show one inflammation or both sinuses, sneezing mucus and low feed intake.

17. – Make a culture of chickens with swollen sinuses, should be positive for ­­­ Avibacterium paragallinarum

18. - The bacterin should give a 100% protection, the protection period is proportional to the adjuvant used in the finished product,

References

1.-Isolation and Identification of Avian  Pathogens. Editorial Committee for The American Association of Avian Pathologists
2.-Clinical Diagnosis by Laboratory Metods Todd, Sanford and Wells
3.-Carlos Rodriguez G Personal experience